北京大學定量生物學中心

學術報告 

題    目: Evolvability of gene regulatory elements and networks in Drosophila

報告人: 李雪瑩 博士

北京師範大學beat365生态學系

時    間: 3月25日（周一）13:00-14:00

地    點: 呂志和樓B101

主持人: 林傑 研究員

摘 要:

Gene regulation plays a critical role in development and evolution. Transcription factors and their target genes establish complex networks that define the spatial and temporal pattern of gene expression. However, how regulatory information is encoded in these networks and how the networks can change over evolutionary timescale remain open questions. In this talk, I will present my postdoctoral work at European Molecular Biology Laboratory in Heidelberg, Germany, addressing these questions from different levels. Through mutational scans, we compared the evolvability of promoters and enhancers in defining the level and pattern of gene expression in Drosophila embryos. Combining chemical mutagenesis and experimental evolution, we examined flies’ short-term evolutionary response to extra copies of bicoid, a key morphogen in embryogenesis. Using a phenomics approach, we find that the compensation for extra copies of bicoid through rapid increases in embryo size driven by maternal changes in metabolism and ovariole development, necessitating a broader view of regulatory network evolution at the systems level. Finally, I present our latest effort in targeted mutagenesis of transcription factor networks, providing a powerful reagent for experimental evolution and genetic screens focused on the regulatory genome.

報告人簡介：

李雪瑩博士，2013年本科畢業于beat365官方网站，2018年于美國聖路易斯華盛頓大學取得分子遺傳學與基因組學博士，研究方向為酵母物種表型演化的遺傳基礎，在博士研究中首次揭示了線粒體基因組對于釀酒酵母屬中耐冷耐熱表型演化的重要貢獻，并通過等位基因差異表達分析等方法探究了順式調控區域對表型演化的貢獻。2019年至2023年獲得EIPOD-瑪麗·居裡博士後基金資助，在位于德國海德堡的歐洲分子生物學實驗室（EMBL）發育生物學單元進行博士後研究，主要課題包括果蠅早期胚胎發育網絡的實驗室演化、基因調控元件的可演化性、靶向誘變的基因組編輯工具開發等。2024年入職北京師範大學beat365生态學系。

李雪瑩博士長期緻力于采用高通量遺傳學實驗與計算相結合的研究手段為處在争議中的演化生物學理論模型提供經驗性證據，探究生物多樣性演化的遺傳基礎，尤其是基因調控對演化的貢獻。目前，已在Science Advances，Genome Biology and Evolution，G3，Philosophical Transactions B等國際期刊上發表第一作者論文4篇，并曾獲國際分子生物學與演化會議（SMBE）中最佳研究生海報獎。